A1062
Sigma
Monoclonal Anti-Rat Kappa & Lambda Light Chains−Alkaline Phosphatase antibody produced in mouse
clone RT-39/RL-6, purified immunoglobulin, buffered aqueous glycerol solution
| Synonym: | Monoclonal Anti-Rat Kappa & Lambda Light Chains |
Description
| Specificity | The product recognizes epitopes located on the rat κ and λ light chains on the various rat immunoglobulin classes and subclasses. The epitope recognized on the κ light chains is shared by both the 1a and 1b allotypes of this molecule. The antibody detects the κ and λ light chains derived from normal serum immunoglobulins or from myeloma proteins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with human, bovine, cat, chicken, dog, goat, horse, mouse, pig, rabbit or sheep immunoglobulins. |
| Physical form | Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide |
| General description | The product is a mixture derived from hybridomas RT-39 and RL-6, secreting monoclonal antibodies against rat κ and λ light chains, respectively. |
Properties
| antibody form | purified immunoglobulin |
| clone | RT-39/RL-6 (monoclonal) |
| form | buffered aqueous glycerol solution |
| mol wt | antigen mol wt 25 kDa |
| application(s) | direct ELISA: 1:12,000 |
| dot blot: 1:20,000-1:80,000 | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 | |
| isotype | IgG1/IgG2a |
| shipped in | wet ice |
| storage temp. | 2-8°C |
Safety
| WGK Germany | 2 |
